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Status |
Public on Aug 21, 2020 |
Title |
batch4_AS_C5_JHG-21 |
Sample type |
RNA |
|
|
Source name |
lung tissue
|
Organism |
Homo sapiens |
Characteristics |
patient: 7337 age: 53 disease state: COPD tissue: transplanted lung tissue
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Treatment protocol |
Not Applicable.
|
Growth protocol |
Not Applicable.
|
Extracted molecule |
total RNA |
Extraction protocol |
“RNeasy Mini” tool kit from Qiagen was applied to extract the total RNA in this study
|
Label |
Biotin
|
Label protocol |
The fragmented cDNA is labeled by terminal deoxynucleotidyl transferase (TdT) using a proprietary DNA Labeling Reagent that is covalently linked to biotin 5.5μg of single-stranded cDNA is required for fragmentation and labeling.
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Hybridization protocol |
GeneChip™ Hybridization, Wash, and Stain Kits were applied in this study.
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Scan protocol |
GeneChip™ Scanner 3000 System was utilized to scan the arrays in this study.
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Data processing |
The analysis tools, including robust multichip array (RMA) algorithm in “affy” (v1.36.1) package and the BrainArray v17 CDF in R (version v3.3.0), were applied to analyze the Microarray expression profile After the above standard data processing, the normalized expression matrix was log2 transformed to get the final expression matrix
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Submission date |
May 22, 2020 |
Last update date |
Aug 22, 2020 |
Contact name |
FENG XU |
E-mail(s) |
feng.xu@hli.ubc.ca
|
Phone |
6047818321
|
Organization name |
UBC
|
Department |
Department of Pathology and Lab Medicine
|
Lab |
Jim Hogg Lab
|
Street address |
Suite 110, 6309 Larkin Dr, 110
|
City |
VANCOUVER |
State/province |
BC |
ZIP/Postal code |
V6T 0C2 |
Country |
Canada |
|
|
Platform ID |
GPL17556 |
Series (1) |
GSE151052 |
The site and nature of terminal bronchiolar destruction in chronic obstructive pulmonary disease (COPD). |
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