|
Status |
Public on Apr 13, 2021 |
Title |
Wt1CreERT2:Rosa26mTmG_3 |
Sample type |
SRA |
|
|
Source name |
epicardial derived cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: heart genotype: Wt1CreERT2:Rosa26mTmG age: 3-6 months
|
Extracted molecule |
total RNA |
Extraction protocol |
Whole heart was digested with Collagenase II through the Langendorff perfusion system. The isolated non-cardiac myocytes fraction was subjected to the Fluorescence Activated Cell Sorting system to generate the EGFP positive epicardial derived cells. Single vialbe EGFP positive cell was loaded onto the 10X Genomics Chromium Single cell Gene expression Solution at the density recommended by the manufacturer and barcoded according to the standard protocol. Libraries were sequenced on an Illumina platform using 75-bp pair-end sequencing on a NovaSeq6000 instrument
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Single cell RNA sample 3
|
Data processing |
Seurat Genome_build: GRCm38 with dTomato and EGFP Supplementary_files_format_and_content: UMI count matrix Supplementary_files_format_and_content: Genes file Supplementary_files_format_and_content: Barcodes file
|
|
|
Submission date |
May 22, 2020 |
Last update date |
Apr 13, 2021 |
Contact name |
Lukas Simon |
Organization name |
Baylor College of Medicine
|
Department |
THINC
|
Street address |
1 Baylor Plaza
|
City |
Houston |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE151084 |
Single cell RNA transcriptome analysis of Epicardial Derived Cells in the Desmoplakin haplo-insufficient Arrhythmogenic Cardiomyopathy mouse |
|
Relations |
BioSample |
SAMN15000071 |
SRA |
SRX8383425 |