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Sample GSM4566105 Query DataSets for GSM4566105
Status Public on Apr 13, 2021
Title Wt1CreERT2:Rosa26mTmG_3
Sample type SRA
 
Source name epicardial derived cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: heart
genotype: Wt1CreERT2:Rosa26mTmG
age: 3-6 months
Extracted molecule total RNA
Extraction protocol Whole heart was digested with Collagenase II through the Langendorff perfusion system. The isolated non-cardiac myocytes fraction was subjected to the Fluorescence Activated Cell Sorting system to generate the EGFP positive epicardial derived cells. Single vialbe EGFP positive cell was loaded onto the 10X Genomics Chromium Single cell Gene expression Solution at the density recommended by the manufacturer and barcoded according to the standard protocol.
Libraries were sequenced on an Illumina platform using 75-bp pair-end sequencing on a NovaSeq6000 instrument
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Single cell RNA
sample 3
Data processing Seurat
Genome_build: GRCm38 with dTomato and EGFP
Supplementary_files_format_and_content: UMI count matrix
Supplementary_files_format_and_content: Genes file
Supplementary_files_format_and_content: Barcodes file
 
Submission date May 22, 2020
Last update date Apr 13, 2021
Contact name Lukas Simon
Organization name Baylor College of Medicine
Department THINC
Street address 1 Baylor Plaza
City Houston
ZIP/Postal code 77030
Country USA
 
Platform ID GPL24247
Series (1)
GSE151084 Single cell RNA transcriptome analysis of Epicardial Derived Cells in the Desmoplakin haplo-insufficient Arrhythmogenic Cardiomyopathy mouse
Relations
BioSample SAMN15000071
SRA SRX8383425

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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