|
| Status |
Public on Sep 30, 2009 |
| Title |
volunteer V22 at time point T1 |
| Sample type |
RNA |
| |
|
| Source name |
A PBMC sample from volunteer V22 at time point T1
|
| Organism |
Homo sapiens |
| Characteristics |
group: NonProtected
cell type: PBMC
|
| Treatment protocol |
PBMC samples are collected at various time points during a malaria vaccine trial
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Follow Affymetrix's protocol
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner
|
| Description |
Gene expression data from a volunteer V22 at time point: the day of the third vaccination;it belongs to NonProtected group
|
| Data processing |
CEL files were normalized using RMA method. This dataset was further normalized with a dataset from Plus2.0 Genechip using quantiles normalization method available in the affy package of bioconductor. Probesets that are absent in all samples are eliminated in the later stage of analysis
|
| |
|
| Submission date |
Sep 29, 2009 |
| Last update date |
Sep 29, 2009 |
| Contact name |
Maryanne Vahey |
| E-mail(s) |
mvahey@hivresearch.org
|
| Phone |
301-251-5058
|
| Fax |
301-762-7460
|
| Organization name |
Walter Reed Army Institute of Research
|
| Department |
|
| Lab |
Laboratory of Functional Genomics
|
| Street address |
1600 East Gude Drive
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE18323 |
Expression data from a malaria vaccine trial (HG-U133A2.0 and U133 Plus 2.0) |
|
