|
| Status |
Public on Sep 05, 2021 |
| Title |
retina_db/+_ZT6_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
neuronal retina, non-diabetic, light-adapted
|
| Organism |
Mus musculus |
| Characteristics |
genotype: db/+
time: ZT6
tissue: Retina
age: Adult
|
| Growth protocol |
Mice (BKS.Cq-Dock7m +/+ Leprdb/J) were kept under light/dark (LD) 12:12 cycles under standard laboratory conditions (illumination with 200 lux at cage level during the day, 20 ± 1°C, water and food ad libitum).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using the Qiagen RNeasy Mini Kit RNA was extracted from retina samples according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
db_day_U2A
Gene expression data from retina of non-diabetic (db/+) mice at ZT6.
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 21 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
|
| |
|
| Submission date |
May 28, 2020 |
| Last update date |
Sep 05, 2021 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23092 |
| Series (1) |
| GSE151350 |
Diurnal gene expression in the retina of a mouse model of diabetic retinopathy |
|
