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Sample GSM457660 Query DataSets for GSM457660
Status Public on Jun 14, 2010
Title ESC rep2
Sample type RNA
 
Source name ESC OG2
Organism Mus musculus
Characteristics potency: pluripotent
cell type: ESC
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Sep 29, 2009
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6887
Series (1)
GSE18330 Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212602 2.044307
ILMN_1212603 0.415909
ILMN_1212605 8.238015
ILMN_1212607 1.050292
ILMN_1212612 0.819425
ILMN_1212614 0.127477
ILMN_1212619 2.877547
ILMN_1212623 0.304181
ILMN_1212625 8.05583
ILMN_1212626 0.382111
ILMN_1212628 0.327301
ILMN_1212632 1.705606
ILMN_1212633 2.343106
ILMN_1212636 10.043866
ILMN_1212637 6.59162
ILMN_1212638 12.600292
ILMN_1212639 3.921453
ILMN_1212644 0.667705
ILMN_1212645 0.309751
ILMN_1212646 9.307683

Total number of rows: 45281

Table truncated, full table size 969 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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