|
| Status |
Public on Jun 04, 2020 |
| Title |
OT1 CD8+ T cells activated with N4 Peptide rep2-HMW IP |
| Sample type |
SRA |
| |
|
| Source name |
OT1 CD8+ T cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6-Tg(TcraTcrb)1100Mjb/J
tissue: Lymph nodes
cell type: Activated CD8+ T cells
purified cellular fraction: HMW RISC Complexes purified by Superose 6 fractionation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy (Qiagen)
CleanTag (Trilink)
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
HMW_IP2
Small RNA sequencing
AGO2 immunoprecipitation carried followed by small RNA sequencing.
|
| Data processing |
QuickMIRSeq miRNA counting, 18-28 bp, trimming 3' adapter TGGAATTCTCGGGTGCCAAGG.
Genome_build: GRCm38.p5
Supplementary_files_format_and_content: GEO_HMWLMWactivated.quickmirseq.grouped_by_mirna.csv: Comma-separated text file with columns: miRNA, sample1, sample2, …, rows have miRNA counts.
|
| |
|
| Submission date |
Jun 01, 2020 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Amy Buck |
| E-mail(s) |
amybucklab@gmail.com, a.buck@ed.ac.uk, sujai.kumar@ed.ac.uk
|
| Organization name |
The University of Edinburgh
|
| Department |
IIIR
|
| Lab |
Buck Lab
|
| Street address |
Ashworth Labs, Charlotte Auerbach Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3FL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE151555 |
Analysis of High Molecular Weight RNA-induced silencing complex (HMW-RISC) in CD8+ T cells identifies miR-7 as a modulator of T cell activation |
|
| Relations |
| BioSample |
SAMN15074813 |
| SRA |
SRX8448779 |
