|
| Status |
Public on Jul 08, 2020 |
| Title |
M3_KO_ln [993269] |
| Sample type |
SRA |
| |
|
| Source name |
lymph node
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype: Aire-/-
treatment: aPD1
tissue: lymph node
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from all samples using MagMAX™-96 for Microarrays Total RNA Isolation Kit (Ambion by Life Technologies) according to manufacturer’s specifications. Genomic DNA was removed using MagMAX™Turbo™DNase Buffer and TURBO DNase from the MagMAX kit listed above (Ambion by Life Technologies).
Strand-specific mRNAseq libraries were prepared from total RNA using the KAPA Stranded mRNA-Seq Kits (Kapa Biosystems).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
993269_1_1_171103_SN7001209_0743_ACBKCVACXX_L5
processed data file: mc38_counts.txt
|
| Data processing |
Array Studio software (OmicSoft) used for reads mapping.
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text file include read count for each sample
|
| |
|
| Submission date |
Jun 04, 2020 |
| Last update date |
Jul 08, 2020 |
| Contact name |
Asiel Arturo Benitez |
| E-mail(s) |
asiel.benitez@regeneron.com
|
| Organization name |
Regeneron Pharmaceuticals, Inc
|
| Department |
Immunology & Inflammation
|
| Street address |
777 Old Saw Mill River Rd, 92-516A
|
| City |
Tarrytown |
| State/province |
New York |
| ZIP/Postal code |
10591 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE151829 |
Absence of central tolerance in Aire-deficient mice synergizes with immune-checkpoint inhibition to enhance antitumor responses [bulk RNA-seq] |
| GSE151831 |
Absence of central tolerance in Aire-deficient mice synergizes with immune-checkpoint inhibition to enhance antitumor responses |
|
| Relations |
| BioSample |
SAMN15104568 |
| SRA |
SRX8473147 |
