|
| Status |
Public on Jun 01, 2022 |
| Title |
FL B cells from BM 109 |
| Sample type |
RNA |
| |
|
| Source name |
B tumor cells purified from follicular lymphoma bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
status: FL patient
tissue: Bone marrow
|
| Treatment protocol |
-
|
| Growth protocol |
FL B cells were purified by FACSARIA strategy from FL bone marrow samples using CD20hiCD44loCD38posIgDnegCD138negCD34neg phenotype.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using Qiazol (Qiagen, Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were amplified according to the small sample labeling protocol (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Following fragmentation, cARN were hybridized on GeneChip Human U133Plus2 arrays
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner Affymetrix 3000
|
| Description |
Cells were purified using FACSARIA sorting
|
| Data processing |
Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC). Normalization was performed sing the Robust Multiarray Analysis (RMA) method. Dataset was then filtering by eliminating PS with no expression in all dataset and/or no gene symbol annotations.
|
| |
|
| Submission date |
Jun 10, 2020 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Celine Pangault |
| E-mail(s) |
celine.pangault@univ-rennes1.fr
|
| Organization name |
Rennes University Hospital
|
| Department |
Medical School of Rennes
|
| Lab |
Hematology INSERM U1236
|
| Street address |
2, avenue du Léon Bernard
|
| City |
RENNES |
| ZIP/Postal code |
35033 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE152215 |
Expression data of human follicular lymphoma (FL) B cells purifed from bone marrow samples (BM) |
|
