|
| Status |
Public on Jun 02, 2021 |
| Title |
Arabidopsis clb5 expanded cotyledon (sample-3) |
| Sample type |
SRA |
| |
|
| Source name |
Arabidopsis expanded cotyledon
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: clb5
tissue: expanded cotyledon
|
| Treatment protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Plant RNA Reagent (Invitrogen, Carlsbad, CA).
RNA-seq libraries were constructed using TruSeq Stranded RNA preparation kit (Illumina, San Diego, CA). All libraries were sequenced on an Illumina Hi-Seq 2500 instrument at the Delaware Biotechnology Institute, Newark, DE.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
clb5_r3
|
| Data processing |
All reads from RNA-seq libraries were trimmed to remove adapter sequences using The quality of sequences was analyzed with Trimmomatic (Bolger et al., 2014). We developed the website and database using the Arabidopsis TAIR10 genome, to store and analyze all libraries; we used these visualization tools extensively for this analysis.
Genome_build: Arabidopsis TAIR10
Supplementary_files_format_and_content: tag count
|
| |
|
| Submission date |
Jun 11, 2020 |
| Last update date |
Jun 02, 2021 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL17639 |
| Series (1) |
| GSE152252 |
Deconvoluting ACS1 retrograde signaling networks that regulate leaf development |
|
| Relations |
| BioSample |
SAMN15210431 |
| SRA |
SRX8549716 |
