|
| Status |
Public on Mar 01, 2010 |
| Title |
IMR-90-miR-29-KD(50nM)-2 |
| Sample type |
RNA |
| |
|
| Source name |
total RNA from IMR-90 cells 48hrs after transfection of 50 nM miR-29 LNA KD oligos
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: fetal lung fibroblast
cell line: IMR-90
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
Transfection was carried out using Lipofectamine™ 2000 (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen) was used for Total RNA isolation.
|
| Label |
standard Affymetrix labeling
|
| Label protocol |
standard Affymetrix labeling
|
| |
|
| Hybridization protocol |
standard Affymetrix hybridization
|
| Scan protocol |
standard Affymetrix scanning
|
| Description |
none
|
| Data processing |
Affymetrix Expression Console software using RMA algorithm (log2 transformed)
|
| |
|
| Submission date |
Oct 20, 2009 |
| Last update date |
Nov 11, 2009 |
| Contact name |
jining lu |
| E-mail(s) |
jining@bu.edu
|
| Phone |
617-638-6180
|
| Fax |
617-536-8093
|
| Organization name |
Boston University
|
| Street address |
715 Albany street R304
|
| City |
BoSTON |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE18651 |
miR-29 targets in human fetal lung fibroblast IMR-90 cells |
|
