|
| Status |
Public on Jun 25, 2020 |
| Title |
array 2-peripheral blood pool 2 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
the number of samples: 2
gender: female
age: 38, 40 years
tissue: peripheral blood
|
| Treatment protocol |
The samples were collected from unrelated and healthy people wihout treatment.
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Life technologies, USA) and purified with the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions. Equal quantities of total RNA from three or two donors were combined to produce pooled samples.
|
| Label |
Hy3™
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
|
| |
|
| Hybridization protocol |
The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual.
|
| Scan protocol |
The slides were scanned using an Axon GenePix 4000 B microarray scanner (Axon Instruments, Foster City, CA).
|
| Data processing |
Data were extracted using GenePix 6.0 software (Axon, USA). Expressed data were normalized using median normalization.
|
| |
|
| Submission date |
Jun 24, 2020 |
| Last update date |
Jun 25, 2020 |
| Contact name |
Weibo Liang |
| E-mail(s) |
liangweibo@gmail.com
|
| Organization name |
Sichuan University
|
| Street address |
Forensic Medicine Building, No.17, Renmin South Road Third Section
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL28766 |
| Series (1) |
| GSE153135 |
microRNA array data for forensically relevant body fluids samples |
|
