|
| Status |
Public on Mar 28, 2003 |
| Title |
BMM_C3H/HeJ_time0.5h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from bone marrow derived macrophage from C3H/HeJ mice after 10ug/ml LPS (S. minnesota) stimulation for 0.5 hours
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
Total RNA from C57BL6/J 17.5 days whole embryo
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Total RNA was extracted using Qiagen RNeasy midi-kit from bone marrow macrophages in-vitro differentiated using human colony stimulator factor-1 (CSF-1) or from C57BL6/J 17.5 whole embryo.
The cDNA derived from the total RNA was labeled using the amino-alyll indirect labeling with Cy3 mono-reactive dye.
|
| |
|
| Submission date |
Feb 28, 2003 |
| Last update date |
Nov 21, 2005 |
| Contact name |
Timothy Ravasi |
| E-mail(s) |
travasi@bioeng.ucsd.edu
|
| Phone |
+1-858-822-4705
|
| Fax |
+1-858-822-4246
|
| URL |
http://chianti.ucsd.edu/travasi
|
| Organization name |
University of California-San Diego
|
| Department |
Department of Bioengineering
|
| Street address |
9500 Gilman Drive
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92093-0412 |
| Country |
USA |
| |
|
| Platform ID |
GPL256 |
| Series (2) |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
same as UNF_VALUE but with flagged values removed |
| CH1_MEAN |
raw signal divided by the area over which the raw signal was measured for Cy3 |
| CH2_MEAN |
raw signal divided by the area over which the raw signal was measured for Cy5 |
| CH1_SD |
standard deviation calculated for the mean signal in Cy3 |
| CH2_SD |
standard deviation calculated for the mean signal in Cy5 |
| CH1_BKD_MEAN |
raw background signal divided by the area over which the raw background signal was measured for Cy3 |
| CH2_BKD_MEAN |
raw background signal divided by the area over which the raw background signal was measured for Cy5 |
| CH1_BKD_SD |
standard deviation calculated for the mean background signal for Cy3 |
| CH2_BKD_SD |
standard deviation calculated for the mean background signal for Cy5 |
| QUALITY |
Quality of the spot in Cy3 and Cy5 channels, "0" = good spots and "1" = bad spots |
| PRE_VALUE |
normalized (Cy3_signal mean-Cy3 bkd mean)/normalized (Cy5 signal mean-Cy5 background mean) |
| UNF_VALUE |
log ratio of PRE_VALUE |
