|
| Status |
Public on Jan 01, 2016 |
| Title |
POLG1 patient P423 |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle POLG1 patient
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 68
age group: old
tissue: skeletal muscle
disease state: POLG4
|
| Treatment protocol |
After biopsy, the muscle samples were immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TRIzol reagent and purified with the Rneasy clean-up kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
150ng muscle RNA was reverse transcribed into cDNA and amplified in a two-round amplification according to the manufacturer's protocol (Affymetrix)
|
| |
|
| Hybridization protocol |
A mixture of cDNA and added hybridization controls was hybridized on Affymetrix HG-U133 Plus 2.0 chips, followed by staining and washing steps in the Affymetrix GeneChip fluidics station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from muscle of patient P423
|
| Data processing |
The data were analyzed with R, a free software program for statistical computing and graphics. Normal linear regression models were used to asses significantly differentially expressed genes between patients and controls.
|
| |
|
| Submission date |
Oct 23, 2009 |
| Last update date |
Jan 01, 2016 |
| Contact name |
An M Voets |
| E-mail(s) |
an.voets@uhasselt.be
|
| Organization name |
Hasselt University
|
| Department |
BIOMED
|
| Street address |
Martelarenlaan 42
|
| City |
Hasselt |
| ZIP/Postal code |
3500 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18715 |
Expression data from POLG1 patients and control subjects muscle |
|
