|
| Status |
Public on Oct 28, 2009 |
| Title |
Nucleus accumbens repeated wd + acute cocaine 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse nucleus accumbens 1 hr
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
agent: 7 treatments 20 mg/kg cocaine-HCl + 7 treatments saline + 1 challenge treatment 20 mg/kg cocaine-HCl, i.p.
age: 8-10 week old
tissue: Bilateral, 1mm-thick, 15-gauge punches, pooled together from 4 animals
|
| Treatment protocol |
Animals were treated daily with either 'saline' (15 treatments saline, i.p.), 'acute' cocaine (14 treatments saline + 1 treatment 20 mg/kg cocaine-HCl, i.p.), 'repeated + acute' cocaine (7 treatments saline + 8 treatments 20 mg/kg cocaine-HCl, i.p.) or 'repeated withdrawal + acute' cocaine (7 treatments 20 mg/kg cocaine-HCl + 7 treatments saline + 1 challenge treatment 20 mg/kg cocaine-HCl, i.p.) and were sacrificed 1 hour after the final treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 1
|
| Data processing |
The data were normalized using quantile normalization with Beadstudio software from illumina
|
| |
|
| Submission date |
Oct 27, 2009 |
| Last update date |
Oct 27, 2009 |
| Contact name |
Ian Sutherland Maze |
| E-mail(s) |
ian.maze@mssm.edu
|
| Phone |
212-659-5934
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Neuroscience
|
| Lab |
Nestler Lab
|
| Street address |
1425 Madison Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE18751 |
Genomic sensitization in response to G9a repression following repeated cocaine exposure |
|
