|
| Status |
Public on Jul 08, 2020 |
| Title |
Normal OSCC-11 |
| Sample type |
RNA |
| |
|
| Source name |
Oral mucosa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Oral squamous cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by lysing the tissues with Isogen (Nippon Gene) after homogenization with a TissueLyser (Qiagen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
500 ng of total RNA was labeled using a GeneChipTM 3' IVT PLUS Reagent Kit (Affymetrix) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
7.5 µg fragmented and labeled cRNA were hybridized for 16h at 45℃ on Human Genome U219 Array Strip. Array Strip was washed and stained in the GeneAtlasTM fluidics station.
|
| Scan protocol |
Array Strip was scanned in the GeneAtlasTM imaging station.
|
| Data processing |
The robust multichip average (RMA) method was used background correction and normalization.
|
| |
|
| Submission date |
Jul 07, 2020 |
| Last update date |
Jul 08, 2020 |
| Contact name |
Koh-ichi Nakashiro |
| E-mail(s) |
nakako@m.ehime-u.ac.jp
|
| Phone |
+81899605393
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE153918 |
Oral squamous cell carcinoma may originate from bone marrow-derived stem cells |
|
