|
| Status |
Public on Oct 30, 2009 |
| Title |
P10 7d SNI; Biological replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Rat spinal dorsal horn
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: spinal cord (ipsilateral dorsal horn)
developmental stage: Neonate (P10)
treatment group: 7 day post sciatic nerve SNI injury
|
| Treatment protocol |
SNI surgery was performed where the tibial and common peroneal branches of the sciatic nerve were tightly ligated with a silk suture and transected distally, while the sural nerve was left intact (Decosterd and Woolf, 2000). In sham-operated controls, the sciatic nerve was exposed but not ligated. Operation performed under isofluorane.
|
| Growth protocol |
Standard laboratory rodent housing, with free access to water and food and were exposed to a 12:12 hour on/off light cycle. Pups left with mothers following surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Global gene expression
|
| Data processing |
The Bioconductor affy library was used to import CEL file data and the gcrma library was used to normalize the raw data.
|
| |
|
| Submission date |
Oct 29, 2009 |
| Last update date |
Oct 29, 2009 |
| Contact name |
Caroline Emma Johnston |
| E-mail(s) |
caroline.johnston@iop.kcl.ac.uk
|
| Organization name |
Kings College London
|
| Street address |
125 Coldharbour Lane
|
| City |
London |
| ZIP/Postal code |
SE5 9NU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE18803 |
Adult and Neonatal dorsal horn gene expression 7 day post sciatic nerve SNI injury |
|
