|
| Status |
Public on Mar 01, 2010 |
| Title |
PMN_mock_60min_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PMN_mock_60min
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell type: neutrophils
|
| Treatment protocol |
Purified human neutrophils were incubated in a 24 well dish for 60 minutes at 37 degrees C and 5% CO2 with or without bacteria at a multiplicity of infection of 10 bacteria per neutrophil.
|
| Growth protocol |
Neutrophils were purified from the whole blood of healthy adult male and female volunteers and used immediately.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the Qiagen RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Chips labeled with biotin according to Manufacturer's protocol
|
| |
|
| Hybridization protocol |
Chips hybridized according to Manufacturer's protocol
|
| Scan protocol |
GS3000
|
| Description |
Gene expression data from neutrophils exposed to RPMI media only for 60 minutes; replicate 2.
|
| Data processing |
Data corrected to 1500 Baseline
|
| |
|
| Submission date |
Oct 29, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Rekha Meyer |
| E-mail(s) |
rmeyer@pathbox.wustl.edu
|
| Phone |
314 362 8853
|
| Organization name |
Washington University School of Medicine
|
| Street address |
4320 Forest Park Av
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63128 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18810 |
Expression data from human neutrophils exposed to uropathogenic and commensal Esherichia coli. |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE86362 |
