|
| Status |
Public on Jul 10, 2023 |
| Title |
SHRSP-1F344 Male 14 weeks normal salt, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Rat 14 weeks old, cardiac tissus, free wall left ventricle
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Rat consomic SHRSP-1F344
tissue: cardiac, left ventricle
Sex: male
age: 14 weeks old
|
| Treatment protocol |
normal salt for 14 weeks
|
| Growth protocol |
All animals were maintained under standard conditions of regular 12h diurnal cycles using an automated light switching device and climate-controlled conditions at a constant room temperature of 22°C. Animals had access to food and water ad libitum. All animal experiments were approved by the responsible local government committee in accordance with the guidelines of the Charité-Universitätsmedizin Berlin and national animal protection guidelines (Landesamt für Gesundheit und Soziales (LAGeSo) Berlin, Germany) for the use of laboratory animals. Male SHRSP and F344 rats were obtained from our colonies at the Forschungseinrichtung für Experimentelle Medizin (FEM), Charité - Universitätsmedizin, Berlin. To develop the consomic strain SHRSP-1F344 (Rat Genome Database (RGD)-ID: SHRSP-Chr 1F344/Rkb), we crossed the SHRSP strain with the F344 strain in accordance with our linkage results and introgressed the whole rat chromosome (RNO)1 from F344 into the SHRSP background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hearts were dissected and arrested in diastole by rinsing in 1 M KCl solution and subsequently blotted dry. The atria were trimmed away and hearts and kidneys were weighed. LV tissue was separated from septum and right ventricle for determination of LV weight. RNA was isolated from the free wall LV by the TRIzol reagent according to the manufacturer's instructions and dissolved in DEPC-treated H2O. The quality and concentration of the purified total RNA were confirmed by spectroscopy.
|
| Label |
Cy3
|
| Label protocol |
biotin-cRNA was performed using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChip according to the manufacturer's instruction
|
| |
|
| Hybridization protocol |
sample hybridization was performed following the WGGEX Direct Hybridization Assay Guide
|
| Scan protocol |
BAR standard
|
| Description |
53.2
direct hybridization assay
|
| Data processing |
The data were normalised using quantile normalisation and the different gene expression analysis were performed using the R packages systemsbio and limma
|
| |
|
| Submission date |
Jul 13, 2020 |
| Last update date |
Jul 10, 2023 |
| Contact name |
Anika Witten |
| E-mail(s) |
anika.witten@uni-muenster.de
|
| Organization name |
University of Muenster
|
| Department |
Institute of Human Genetics, Department Genetic Epidemiology
|
| Street address |
Domagkstr. 3
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6101 |
| Series (2) |
| GSE154326 |
Cpxm2 as a novel candidate for cardiac hypertrophy and failure in hypertension [Illumina] |
| GSE154327 |
Cpxm2 as a novel candidate for cardiac hypertrophy and failure in hypertension |
|
