|
| Status |
Public on Sep 29, 2021 |
| Title |
K_50uM_DEAB_t_3hr |
| Sample type |
RNA |
| |
|
| Source name |
whole embryo
|
| Organism |
Xenopus laevis |
| Characteristics |
genotype: wildtype
treatment group: 50µM DEAB for 2hrs , t=3hr after wash
|
| Treatment protocol |
The experimental design involves treating embryos for 2 hours from late blastula (st. 9.5) to early gastrula (st. 10.25) and collecting samples every 1.5 hours after terminating and washing the treatment. Embryos were treated with RA (10 nM) or DEAB (50 µM). For each biological repeat, all treatments, controls, and time points were collected from a single fertilization, from the eggs (clutch) of a single female.
|
| Growth protocol |
Embryos were obtained by in vitro fertilization, incubated in 0.1% MBSH and staged according to Nieuwkoop and Faber(Nieuwkoop and Faber, 1967).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample, 5-10 staged embryos were collected and stored at -80°C. RNA purification was performed using the Bio-Rad Aurum Total RNA Mini Kit (according to the manufacturer's instructions). RNA samples were used for cDNA synthesis using the Bio-Rad iScriptTM Reverse Transcription Supermix for RT-qPCR kit (according to the manufacturer's instructions).
|
| Label |
Eva Green
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
Relative gene expression was determined by the ΔΔCt method. Gapdh was used as a housekeeping gene for normalization of the data.
|
| |
|
| Submission date |
Jul 14, 2020 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Rajanikanth Vadigepalli |
| E-mail(s) |
rajanikanth.vadigepalli@jefferson.edu
|
| Organization name |
THOMAS JEFFERSON UNIVERSITY
|
| Street address |
1020 LOCUST ST RM 314
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL28865 |
| Series (2) |
| GSE154407 |
Robustness in the feedback control of the retinoic acid network response to environmental disturbances (BioMark-qRT-PCR) |
| GSE154408 |
Asymmetric robustness in the feedback control of the retinoic acid network response to environmental disturbances |
|
