|
| Status |
Public on Mar 07, 2011 |
| Title |
untreated neurons UT 2 |
| Sample type |
RNA |
| |
|
| Source name |
untreated neurons
|
| Organism |
Rattus norvegicus |
| Characteristics |
agent: untreated (control)
cell type: cortical neurons
|
| Treatment protocol |
untreated
|
| Growth protocol |
cortical neurons were isolated from d18 Sprague-Dawley rat embryos and grown for 3 days in NB-N2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction using the QIAGEN Rneasy Mini Kit was performed according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on Rat Gene 1.0 ST array. GeneChips were washed in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affymetrix GCOS software
|
| Description |
gene expression data from untreated neurons
|
| Data processing |
JMP genomics v4.0 software, median based inter-array normalization
|
| |
|
| Submission date |
Nov 03, 2009 |
| Last update date |
Mar 07, 2011 |
| Contact name |
Marie Emeline Benoit |
| E-mail(s) |
mbenoit@uci.edu
|
| Organization name |
UC Irvine
|
| Department |
Molecular Biology and Biochemistry
|
| Lab |
Tenner lab
|
| Street address |
2419 McGaugh Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE18860 |
Regulation of neuronal gene and miRNA expression by the complement protein C1q associated with neuroprotection |
|
