|
| Status |
Public on Nov 04, 2009 |
| Title |
Reference tumor 5 |
| Sample type |
RNA |
| |
|
| Source name |
Reference primary tumor sample
|
| Organism |
Homo sapiens |
| Characteristics |
age: 46
miller-payne response: n/a
p53 status: n/d
er/pr/her2 status: pos/pos/neg
grade: II
brca genotype: Unknown
|
| Treatment protocol |
Two 5μ frozen sections were stained with hematoxylin and eosin, manually scraped to remove normal stroma and enrich for tumor cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Absolutely RNA Nanoprep kit (Stratagene).
|
| Label |
Biotin
|
| Label protocol |
First round linear amplification was performed using RiboAmp HS kit (Arcturus). Biotinylated cRNA was prepared by in vitro transcription using the Affymetrix IVT kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on GeneChip Human U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from reference primary breast tumor
|
| Data processing |
Expression values were calculated with the RMA algorithm.
|
| |
|
| Submission date |
Nov 03, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Aron C Eklund |
| Organization name |
Technical University of Denmark
|
| Department |
Center for Biological Sequence Analysis
|
| Street address |
Kemitorvet, Building 208
|
| City |
Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18864 |
Tumor expression data from neoadjuvant trial of cisplatin monotherapy in triple negative breast cancer patients |
|
| Relations |
| Reanalyzed by |
GSE119087 |
