|
| Status |
Public on Jul 01, 2021 |
| Title |
17_DQ16A_Emanual_9-12-19_(Clariom_S_Mouse) |
| Sample type |
RNA |
| |
|
| Source name |
AF D+Q
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Annulus Fibrosus
genotype: C57BL/6
age: 23-month-old
|
| Treatment protocol |
N.A.
|
| Growth protocol |
NP and AF tissues were dissected from lumbar discs of 23M Veh (n=5) and D+Q (n=4) BL6 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pooled tissue from single animal served as an individual sample. Samples were homogenized and total RNA was extracted using RNeasy® Mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Fragmented biotin labeled cDNA was synthesized using the GeneChip WT Plus kit according to ABI protocol (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Gene chips (Mouse Clariom S) were hybridized with fragmented and biotin-labeled cDNA. Arrays were washed and stained with GeneChip hybridization wash & stain kit
|
| Scan protocol |
scanned on an Affymetrix Gene Chip Scanner 3000 7G, using Command Console Software.
|
| Description |
Gene expression data from 23-month-old annulus fibrosus C57BL/6 mouse treated with Dasatinib and Quercetin combination.
|
| Data processing |
Chp files were generated by sst-rma normalization from Affymetrix cel file using Expression Console Software. Experimental group was compared with control group by using Transcriptome array console 4.0 software. The SST-RMA algorithm was used for data normalization.
|
| |
|
| Submission date |
Jul 17, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Emanuel José Novais |
| E-mail(s) |
emanuel.j.novais@gmail.com
|
| Organization name |
Thomas Jefferson
|
| Department |
Department of Orthopaedic Surgery
|
| Street address |
1025 Walnut St., Suite 511 College Bldg.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE154619 |
Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice |
|
