|
| Status |
Public on Jul 01, 2024 |
| Title |
genomic DNA from myoblast sample 2 |
| Sample type |
genomic |
| |
|
| Source name |
myoblast sample 2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
sample type: Cultured Myoblasts
age (yr): 75.5
participant id: sse2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from the muscle tissue using QIAamp DNA mini kit (Qiagen) and from the cultured myoblasts using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma), both according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
myoblast sample
|
| Data processing |
R v3.4.2
|
| |
|
| Submission date |
Jul 22, 2020 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Elie Antoun |
| Organization name |
University of Oxford
|
| Department |
CAMS China Oxford Institute (CAMS-COI
|
| Street address |
Nuffield Department of Medicine
|
| City |
Oxford |
| State/province |
United Kingdom |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE154946 |
Epigenome analysis of muscle tissue and paired cultured myoblasts from the Hertfordshire Sarcopenia Study extension |
|
