|
Status |
Public on Feb 02, 2011 |
Title |
small airways, smoker 328 |
Sample type |
RNA |
|
|
Source name |
airway epithelial cells obtained by bronchoscopy and brushing
|
Organism |
Homo sapiens |
Characteristics |
age: 41 sex: M ethnic group: black smoking status: smoker, 18 pack-years
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
no additional information
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Nov 12, 2009 |
Last update date |
Feb 02, 2011 |
Contact name |
Yael Strulovici-Barel |
E-mail(s) |
yas2003@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Department of Genetic Medicine
|
Lab |
Crystal
|
Street address |
1300 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE11784 |
Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking |
GSE19667 |
Threshold of Biologic Response of the Small Airway Epithelium to Low Levels of Tobacco Smoke |
|