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Sample GSM470010 Query DataSets for GSM470010
Status Public on Feb 02, 2011
Title small airways, smoker 353
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 49
sex: M
ethnic group: black
smoking status: smoker, 35 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description no additional information
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 12, 2009
Last update date Feb 02, 2011
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (2)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE19667 Threshold of Biologic Response of the Small Airway Epithelium to Low Levels of Tobacco Smoke

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1408.63 P 9.4506e-05
AFFX-BioB-M_at 2479.03 P 4.42873e-05
AFFX-BioB-3_at 1450.04 P 4.42873e-05
AFFX-BioC-5_at 4135.17 P 5.16732e-05
AFFX-BioC-3_at 5964.98 P 4.42873e-05
AFFX-BioDn-5_at 9940.64 P 4.42873e-05
AFFX-BioDn-3_at 17301.3 P 4.42873e-05
AFFX-CreX-5_at 60638 P 5.16732e-05
AFFX-CreX-3_at 61129.3 P 4.42873e-05
AFFX-DapX-5_at 58.2213 A 0.131361
AFFX-DapX-M_at 31.0222 A 0.41138
AFFX-DapX-3_at 6.3504 A 0.904333
AFFX-LysX-5_at 4.24832 A 0.804754
AFFX-LysX-M_at 15.362 A 0.60308
AFFX-LysX-3_at 55.448 A 0.0676785
AFFX-PheX-5_at 16.7031 A 0.65947
AFFX-PheX-M_at 4.80915 A 0.960352
AFFX-PheX-3_at 37.5963 A 0.39692
AFFX-ThrX-5_at 45.3414 A 0.52976
AFFX-ThrX-M_at 15.5822 A 0.57405

Total number of rows: 54675

Table truncated, full table size 1639 Kbytes.




Supplementary file Size Download File type/resource
GSM470010.CEL.gz 7.8 Mb (ftp)(http) CEL
GSM470010.CHP.gz 12.9 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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