|
| Status |
Public on Mar 31, 2010 |
| Title |
miRNA_neutrophils_after exercise_03 |
| Sample type |
RNA |
| |
|
| Source name |
Human netrophils after 20 min bout of exercise
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: neutrophils
|
| Treatment protocol |
Blood sample was drawn before and immediately after 20 min of exercise.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Neutrophils were isolated from EDTA treated peripheral blood using OptiPrep Density Gradient Medium (SIGMA). Using Wright-Giemsa stain, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® (Gibco BRL Life Technologies, Rockville, MD).
|
| Label |
Cy3
|
| Label protocol |
One hundred ng of total RNA was labeled with the fluorescent dye Cyanine 3-pCp (Cy3) using the miRNA Labeling Reagent and Hybridization Kit (Agilent Technologies, Palo Alto, CA) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Cy3-labeled RNA from each sample was hybridized to an Agilent Human miRNA Version 2 Microarray.
|
| Scan protocol |
The hybridized array was then washed and scanned according to Agilent specifications and data was extracted from the scanned image using Feature Extraction version 10.2 (Agilent Technologies).
|
| Description |
gene expression after-exercise
|
| Data processing |
The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
|
| |
|
| Submission date |
Nov 12, 2009 |
| Last update date |
Mar 31, 2010 |
| Contact name |
Shlomit Radom-Aizik |
| E-mail(s) |
saizik@uci.edu
|
| Phone |
949-824-2584
|
| Organization name |
University of California, Irvine
|
| Department |
Pediatrics
|
| Lab |
Pediatric Exercise and Genomics Research Center
|
| Street address |
101 Academy
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE18999 |
Effects of exercise on miRNA expression level in human neutrophils |
|
