|
Status |
Public on Mar 31, 2010 |
Title |
miRNA_neutrophils_before exercise_07 |
Sample type |
RNA |
|
|
Source name |
Human netrophils before exercise
|
Organism |
Homo sapiens |
Characteristics |
cell type: neutrophils
|
Treatment protocol |
Blood sample was drawn before and immediately after 20 min of exercise.
|
Extracted molecule |
total RNA |
Extraction protocol |
Neutrophils were isolated from EDTA treated peripheral blood using OptiPrep Density Gradient Medium (SIGMA). Using Wright-Giemsa stain, we determined that this approach to neutrophil isolation consistently yielded ≥ 98% purification. Total RNA was extracted using TRIzol® (Gibco BRL Life Technologies, Rockville, MD).
|
Label |
Cy3
|
Label protocol |
One hundred ng of total RNA was labeled with the fluorescent dye Cyanine 3-pCp (Cy3) using the miRNA Labeling Reagent and Hybridization Kit (Agilent Technologies, Palo Alto, CA) following the manufacturer’s protocol.
|
|
|
Hybridization protocol |
Cy3-labeled RNA from each sample was hybridized to an Agilent Human miRNA Version 2 Microarray.
|
Scan protocol |
The hybridized array was then washed and scanned according to Agilent specifications and data was extracted from the scanned image using Feature Extraction version 10.2 (Agilent Technologies).
|
Description |
gene expression before-exercise
|
Data processing |
The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
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|
|
Submission date |
Nov 12, 2009 |
Last update date |
Mar 31, 2010 |
Contact name |
Shlomit Radom-Aizik |
E-mail(s) |
saizik@uci.edu
|
Phone |
949-824-2584
|
Organization name |
University of California, Irvine
|
Department |
Pediatrics
|
Lab |
Pediatric Exercise and Genomics Research Center
|
Street address |
101 Academy
|
City |
Irvine |
State/province |
CA |
ZIP/Postal code |
92617 |
Country |
USA |
|
|
Platform ID |
GPL7731 |
Series (1) |
GSE18999 |
Effects of exercise on miRNA expression level in human neutrophils |
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