|
| Status |
Public on Jul 30, 2020 |
| Title |
NP4 |
| Sample type |
SRA |
| |
|
| Source name |
Splenic plasmablast
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
day post infection (dpi): 14
preganancy status: Non-pregnant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10,000 plasmablasts (CD138+B220int 229 ) were sorted from the spleens of pregnant and Pregnancy Confounds Influenza-Specific Humoral Responses Pregnancy Confounds Influenza-Specific Humoral Responses
Single-end RNA sequencing was performed on samples in duplicate to increase sequencing depth (101 base pair reads).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
The Rsubread package align function was used to align single end reads to the Mus musculus genome (GRCm38.93), BAM file created after alignment
Duplicate BAM files were merged together
Genes were counted using the featureCounts function of Rsubread
Genome_build: GRCm38.93
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample in cvs file
|
| |
|
| Submission date |
Jul 29, 2020 |
| Last update date |
Jul 30, 2020 |
| Contact name |
Lisa Mills |
| E-mail(s) |
lisa.k.mills87@gmail.com
|
| Organization name |
CDC
|
| Street address |
1600 Clifton Road
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30021 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE155388 |
Pregnancy Downregulates Plasmablast Metabolic Gene Expression Following Influenza without Altering Long-Term Antibody Function |
|
| Relations |
| BioSample |
SAMN15668993 |
| SRA |
SRX8845603 |
