|
Status |
Public on Jul 01, 2012 |
Title |
Plasmodium falciparum, parasite control, A |
Sample type |
RNA |
|
|
Source name |
Plasmodium falciparum FCR3, parasite control
|
Organism |
Plasmodium falciparum |
Characteristics |
strain: FCR3 csa adhesion: yes developmental stage: 24h after late trophozoite stage
|
Treatment protocol |
Prior to each experiment, FCR3CSA parasites were selected for chondroitin sulfate A (CSA) adhesion by panning. Both strains were constantly synchronized with 5% sorbitol and mature trophozoite-infected erythrocytes (iRBCs) were purified by magnetic cell sorting LD columns (MACS; Miltenyi Biotec). After 24 hours culture of FCR3CSA-infected erythrocytes at 37ÂșC in 5% CO2, RNA was extracted.
|
Growth protocol |
FCR3CSA was maintained in continuous culture as described by Trager and Jensen.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated with Trizol according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix labeling protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix Hybridization Protocol.
|
Scan protocol |
Standard Affymetrix Scanning Protocol.
|
Description |
Control culture.
|
Data processing |
Signals were normalized using GC-RMA.
|
|
|
Submission date |
Nov 13, 2009 |
Last update date |
Jul 01, 2012 |
Contact name |
Evelyn Boettger |
E-mail(s) |
evelyn-boettger@hotmail.de
|
Organization name |
Institute of Tropical Medicine
|
Street address |
Wilhelmstrasse 27
|
City |
Tuebingen |
ZIP/Postal code |
72074 |
Country |
Germany |
|
|
Platform ID |
GPL1321 |
Series (1) |
GSE19010 |
Gene expression profiling of Plasmodium falciparum after co-culture with NK cells |
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