|
| Status |
Public on Jan 22, 2010 |
| Title |
Pou5f1-GFP MEF, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblast (MEF) haboring the GFP transgene driven by Pou5f1 promoter
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblast (MEF) haboring the GFP transgene driven by Pou5f1 promoter
|
| Treatment protocol |
n.a.
|
| Growth protocol |
MEFs were cultured at 37°C with 5% CO2. MEFs were maintained in 15% FBS-DMEM containing 20 ug ml-1 gentamicin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and purified with RNeasy minikit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 500 ng of total RNA according to the standard protocol (TotalPrep RNA Amplification Kit, Instruction Manual, Catalog #IL1791, Illumina).
|
| |
|
| Hybridization protocol |
1.5 ug of cRNA were hybridized to Illumina Mouse-6 Expression BeadChip v1.1 for 20 hr at 58oC on Ilumina Hybridization Oven.
|
| Scan protocol |
Illumina Mouse-6 Expression BeadChip v1.1 were washed and stained with streptavidin-Cy3 before scanning using the Illumina Bead Reader.
|
| Description |
Pou5f1-GFP transgenic mice were from the Jackson Laboratory, Stock No 004654. Pou5f1-GFP MEFs were prepared from E13.5 embryos obtained from the intercross between male Pou5f1-GFP mice and female wild-type C57BL/6J mice.
|
| Data processing |
The data were analyzed using Bead Array suite software. Rank invariant normalization method was chosen to obtain the signal intensity.
|
| |
|
| Submission date |
Nov 13, 2009 |
| Last update date |
Jan 22, 2010 |
| Contact name |
Dominic Jian Chien Heng |
| E-mail(s) |
hengjcd99@gis.a-star.edu.sg
|
| Phone |
65-96734683
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Stem Cell and Developmental Biology
|
| Lab |
Stem Cell and Development Biology 6
|
| Street address |
60 Biopolis Street #02-01, Genome Building
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6105 |
| Series (3) |
| GSE19021 |
Global gene expression analyses of the Nr5a2 reprogrammed cells |
| GSE19022 |
Global gene expression analysis of OSKM / N2SKM- infected MEFs over time course |
| GSE19023 |
The Nuclear Receptor Nr5a2 can replace Oct4 in the Reprogramming of Murine Somatic Cells to Pluripotent Cells |
|
