|
| Status |
Public on Nov 02, 2020 |
| Title |
CD3 enriched T cells DONOR6_(miRNA-3_0) |
| Sample type |
RNA |
| |
|
| Source name |
CD3 positive T cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: unknown
|
| Treatment protocol |
CD 3positive T-cells from five healthy donors were immunomagnetically purified from peripheral blood. T-PLL cells were FACS sorted from ten patients with T-PLL.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
One μg of total RNA including miRNA from each sample was labeled with biotin using the FlashTag Biotin HSR Kit (Genisphere, Hatfield, PA).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufactorers' recommendations (GeneChip miRNA 3.0 arrays (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Scanning was performed according to the manufactorers' recommendations (GeneChip miRNA 3.0 arrays (Affymetrix, Santa Clara, CA).
|
| Description |
CD3 enriched T cells
miRNA expression profiling
|
| Data processing |
The data were extracted from the images, background corrected (RMA), quantile-normalized, summarized (median polish), and log2-transformed with Partek Genomic Suite (Partek Incorporated, St. Louis, MO, USA). Differentially expressed miRNAs were determined by Student´s t-test and p-values were corrected for multiple testing.
|
| |
|
| Submission date |
Aug 05, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Rene Scholtysik |
| E-mail(s) |
rene.scholtysik@uni-due.de
|
| Organization name |
University Hospital Essen
|
| Department |
IFZ
|
| Street address |
Virchowstrasse 173
|
| City |
Essen |
| ZIP/Postal code |
45127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE155746 |
Ant-leukemic effect of CDK9 inhibition in T-cell prolymphocytic leukemia (microRNA) |
|
