|
| Status |
Public on Nov 10, 2021 |
| Title |
Crown_2 |
| Sample type |
SRA |
| |
|
| Source name |
Crown
|
| Organism |
Triticum aestivum |
| Characteristics |
tissue: crown tissue colleected at winter dormancy stage
|
| Treatment protocol |
samples were collected at different growth stages.
|
| Growth protocol |
Winter wheat Shimai 22 was grown in the fileld at Wheat Experimental Station of Henan Normal University. The crown tissues were collected at different stages, and snap frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent according to the manufacture's protocol.
Small RNA libraries were prepared by following the BGISEQ's protocol.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Getting rid of low quality reads
Getting rid of reads which proportion of N
Getting rid of reads with 5' primer contaminants and reads without the insert fragments
Getting rid of reads without 3' primer
Getting rid of reads with poly A
Getting rid of reads shorter than 18nt
Supplementary_files_format_and_content: sequences and count values
|
| |
|
| Submission date |
Aug 10, 2020 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Miao Zhao |
| E-mail(s) |
Miao_Zhao_95@126.com
|
| Phone |
15837359861
|
| Organization name |
Henan Normal University
|
| Department |
Life sciences college
|
| Street address |
Jianshe east road
|
| City |
Xinxiang |
| State/province |
Henan |
| ZIP/Postal code |
453007 |
| Country |
China |
| |
|
| Platform ID |
GPL26077 |
| Series (1) |
| GSE155963 |
Temporal expression study of microRNAs in crown tissues of winter wheat grown under natural growth condition |
|
| Relations |
| BioSample |
SAMN15773553 |
| SRA |
SRX8915516 |
