|
Status |
Public on Feb 28, 2021 |
Title |
HCC1395 GapmeR 1 N1 |
Sample type |
RNA |
|
|
Source name |
HCC1395 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCC1395 cells treatment: GapmeR 1
|
Treatment protocol |
cultured cells treated for 48hours with GapmeRs
|
Growth protocol |
The cell lines were obtained from the American Type Culture Collection (ATCC) and were cultured as per ATCC guidelines
|
Extracted molecule |
total RNA |
Extraction protocol |
cells were collected in TRIzol and total RNA purified using a PureLink RNA kit (Invitrogen Thermo Fisher Scientific) and incorporating a DNAse treatment step following the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Affymetrix WT Plus kit and biotin allonamide triphosphate
|
|
|
Hybridization protocol |
16h at 45oC, Affymetrix Human Gene 2.0 ST microarray platform
|
Scan protocol |
Affymetrix genechip scanner 3000 (Scanner#4)
|
Data processing |
RMA expression value derived from Expression Console software
|
|
|
Submission date |
Aug 12, 2020 |
Last update date |
Mar 01, 2021 |
Contact name |
Paola Marcato |
E-mail(s) |
paola.marcato@dal.ca
|
Phone |
9024944239
|
Organization name |
Dalhousie University
|
Department |
Pathology
|
Street address |
11F11, 5850 College Street
|
City |
Halifax |
State/province |
Nova Scotia |
ZIP/Postal code |
B3H 4R2 |
Country |
Canada |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE156114 |
The effect of lncRNA PART1 knockdown on gene expression in HCC1806 and HCC1395 cells |
|