|
| Status |
Public on Aug 15, 2020 |
| Title |
CD138 - ATAC_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
CD34-CD138+
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD34-CD138+
strain: C57BL/6
age: 12 Week
Sex: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were isolated from mouse colon using Collagenase VIII and Dnase I, followed by AriaIII flow cytometer sorting with CD45/Epcam/CD31 negative, gp38 positive and cell subset specific Abs.
Libraries were prepared according to Novazyme's ATAC instructions accompanying the Kit TD501.
|
| |
|
| Library strategy |
ATAC-seq |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Basecalls performed using Bcl2fastq
ATAC-seq reads were further Analyzed using esATAC
Genome_build: mm10
Supplementary_files_format_and_content: Bigwig files were generated using esATAC
Supplementary_files_format_and_content: Bed files were generated using esATAC and BedTools
|
| |
|
| Submission date |
Aug 14, 2020 |
| Last update date |
Nov 23, 2020 |
| Contact name |
Hongxiang Sun |
| E-mail(s) |
sun758426@sjtu.edu.cn
|
| Organization name |
Shanghai Jiaotong University
|
| Department |
Shanghai Institute of Immunology
|
| Lab |
Bing Su
|
| Street address |
280 South Chongqing Rd.
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE156245 |
Map3k2-regulated intestinal stromal cells define a distinct intestine stem cell niche for tissue repair |
|
| Relations |
| BioSample |
SAMN13547475 |
| SRA |
SRX7352078 |
