|
| Status |
Public on Mar 23, 2021 |
| Title |
HTBE-IFN-1 |
| Sample type |
SRA |
| |
|
| Source name |
tracheobronchial epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: tracheobronchial epithelial cells
treatment: Interferon
replicate: 1
|
| Treatment protocol |
Samples were stimulated with 100 IU/ml universal type I interferon or a mock treatment. After 1 hour, cells were washed and then incubated with medium at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
8 hours after stimulation, cell extracts were collected with Trizol and total RNA was extracted using RNeasy Mini Kit (Qiagen).
Strand-specific rRNA-depleted sequencing libraries were produced according to standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Raw sequencing reads were aligned to the human hg38 genome with STAR
Read counts per gene were calculated using FeatureCounts
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited raw read counts per gene
|
| |
|
| Submission date |
Aug 14, 2020 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Max Chang |
| E-mail(s) |
mchang@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE156295 |
Type I interferon response in human HTBE and A549 cells |
|
| Relations |
| BioSample |
SAMN15824118 |
| SRA |
SRX8949759 |
