|
Status |
Public on Mar 23, 2021 |
Title |
HTBE-IFN-1 |
Sample type |
SRA |
|
|
Source name |
tracheobronchial epithelial cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: tracheobronchial epithelial cells treatment: Interferon replicate: 1
|
Treatment protocol |
Samples were stimulated with 100 IU/ml universal type I interferon or a mock treatment. After 1 hour, cells were washed and then incubated with medium at 37 °C.
|
Extracted molecule |
total RNA |
Extraction protocol |
8 hours after stimulation, cell extracts were collected with Trizol and total RNA was extracted using RNeasy Mini Kit (Qiagen). Strand-specific rRNA-depleted sequencing libraries were produced according to standard Illumina protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
Raw sequencing reads were aligned to the human hg38 genome with STAR Read counts per gene were calculated using FeatureCounts Genome_build: hg38 Supplementary_files_format_and_content: tab-delimited raw read counts per gene
|
|
|
Submission date |
Aug 14, 2020 |
Last update date |
Mar 23, 2021 |
Contact name |
Max Chang |
E-mail(s) |
mchang@ucsd.edu
|
Organization name |
University of California, San Diego
|
Street address |
9500 Gilman Drive
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platform ID |
GPL20301 |
Series (1) |
GSE156295 |
Type I interferon response in human HTBE and A549 cells |
|
Relations |
BioSample |
SAMN15824118 |
SRA |
SRX8949759 |