|
| Status |
Public on Jul 01, 2024 |
| Title |
FOP1 |
| Sample type |
SRA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
disease state: fibrodysplasia ossificans progressiva
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1 ml whole blood from each subject was collected in the occipital sinus in EDTA tubes. After centrifugation, 250 μL of blood was diluted in a tube containing 750 μL of TRIzol® reagent (Sigma, USA, T9294). Total RNA was extracted according to the manufacturer's instructions and resuspended in 50 μL of H2O.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RNA samples from three FOP patients and three health controls were sequenced using a standard Illumina protocol.
Reads were mapped to a human genome (hg19) by using hisat2 (v2.1).
Gene counts were estimated by HTSeq (0.11).
Genome_build: hg19
Supplementary_files_format_and_content: text with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Aug 19, 2020 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Dunmin She |
| E-mail(s) |
sdm1979@126.com
|
| Phone |
+862166111061
|
| Organization name |
Northern Jiangsu People’s Hospital
|
| Department |
Endocrinology
|
| Street address |
98，Western Nantong
|
| City |
Yangzhou |
| State/province |
CHINA |
| ZIP/Postal code |
225000 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE156459 |
RNA-sequencing of peripheral blood in FOP patients |
| GSE156466 |
RNA-Seq of peripheral blood in FOP patients |
|
| Relations |
| BioSample |
SAMN15856223 |
| SRA |
SRX8969345 |
