|
| Status |
Public on Jul 01, 2024 |
| Title |
control 3 |
| Sample type |
SRA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
disease state: Health control
treatment: untreated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were isolated from PB using Ficoll Hypaque gradient centrifugation
PCR products were purified and library quality was assessed on the Agilent Bioanalyzer 2100 system. Then, the library preparations were sequenced on an Illumina platform and 125 bp /150 bp paired-end reads were generated.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
single-cell RNA
|
| Data processing |
Reads mapping, sample merge and single-cell counting was performed using the Cell Ranger Single-Cell Software Suite (10x Genomics).
Cell ranger count was used to align samples to the reference genome (GRCh38, download from 10xgenomics.com).
The Seurat package in R was used for subsequent analysis. For quality control, nuclei with mitochondrial content >15% were removed.
Principal component analysis was performed prior to clustering and the first 20 principal components were used based on the Elbow Plot.
Genome_build: GRCh38
|
| |
|
| Submission date |
Aug 19, 2020 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Dunmin She |
| E-mail(s) |
sdm1979@126.com
|
| Phone |
+862166111061
|
| Organization name |
Northern Jiangsu People’s Hospital
|
| Department |
Endocrinology
|
| Street address |
98，Western Nantong
|
| City |
Yangzhou |
| State/province |
CHINA |
| ZIP/Postal code |
225000 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE156464 |
Single cell RNA-sequencing of PBMCs in FOP patients |
| GSE156466 |
RNA-Seq of peripheral blood in FOP patients |
|
| Relations |
| BioSample |
SAMN15856724 |
| SRA |
SRX8969371 |
