|
| Status |
Public on Mar 15, 2021 |
| Title |
Sample 15_SIKmut-head-fed1 |
| Sample type |
SRA |
| |
|
| Source name |
SIKmut-head-fed
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: sik2 mutant fruit fly
age: 3-5 days old
tissue: adult fly head
treatment: fed
|
| Treatment protocol |
16h fasting
|
| Growth protocol |
standard fly media
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
total RNA extracted with Qiagen Rneasy kit
polyA RNA isolated and library prepared with NEBnext Ultra kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
q2
processed data file:
3_fed-head-KO_gene_exp_sample13-15.diff
|
| Data processing |
.diff file from cuffdiff comparing the samples indicated in the file name:
MiSeq Generation of fastq files
tophat2 alignment
cuffdiff Differential Gene Expression
.txt file from DESeq2 comparing thhe samples indicated in the file name:
MiSeq Generation of fastq files
STAR alignment
DESeq2 Differential Gene Expression
Genome_build: dm3 (.diff files); dm6 (DESeq2 output)
Supplementary_files_format_and_content: .diff file from cuffdiff or .txt file from DESeq2 comparing the samples indicated in the file name
|
| |
|
| Submission date |
Aug 20, 2020 |
| Last update date |
Mar 15, 2021 |
| Contact name |
Ezra Wiater |
| E-mail(s) |
wiater@salk.edu
|
| Phone |
(858)453-4100
|
| Organization name |
Salk Institute For Biological Studies
|
| Department |
PBL
|
| Lab |
Montminy
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE156577 |
Drosophila CRTC promotes starvation resistance by modulating one-carbon metabolism and insulin signaling |
|
| Relations |
| BioSample |
SAMN15867728 |
| SRA |
SRX8978763 |
