|
| Status |
Public on Aug 19, 2023 |
| Title |
A549 cells, SEMA5A_ECD, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549 non-small cell lung cancer cell line
transfection: SEMA5A_ECD
|
| Treatment protocol |
Cells were transfected with different domains of SEMA5A expressing plasmids and control plasmid by jetPRIME transfection reagent (Polyplus-transfection, Illkirch, France) according to the manufacturer’s protocol.
|
| Growth protocol |
A549 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (GIBCO; Thermo Fisher, Waltham, MA, USA) with 10% fetal bovine serum (FBS) (Millipore, US Origin, Korea Eligible) and 1% penicillin-streptomycin (GIBCO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured cells using NucleoZOL reagent (Machery-Nagel, Düren, Germany) according to the manufacturer’s protocol
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Invitrogen Illumina™ TotalPrep™ RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 6
replicate 3
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 28, 2020 |
| Last update date |
Aug 19, 2023 |
| Contact name |
Liang-Chuan Lai |
| E-mail(s) |
llai@ntu.edu.tw
|
| Phone |
886-2-23123456
|
| Organization name |
National Taiwan University
|
| Department |
Physiology
|
| Street address |
1 Jen-Ai Road, Sec 1
|
| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
10051 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE157062 |
Microarray analysis of lung cancer cells gene expression in overexpressing different semaphorin 5a domains |
|
