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Sample GSM4751850 Query DataSets for GSM4751850
Status Public on Aug 19, 2023
Title A549 cells, SEMA5A_Full, rep2
Sample type RNA
 
Source name A549 cells
Organism Homo sapiens
Characteristics cell line: A549 non-small cell lung cancer cell line
transfection: SEMA5A_Full
Treatment protocol Cells were transfected with different domains of SEMA5A expressing plasmids and control plasmid by jetPRIME transfection reagent (Polyplus-transfection, Illkirch, France) according to the manufacturer’s protocol.
Growth protocol A549 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (GIBCO; Thermo Fisher, Waltham, MA, USA) with 10% fetal bovine serum (FBS) (Millipore, US Origin, Korea Eligible) and 1% penicillin-streptomycin (GIBCO)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cultured cells using NucleoZOL reagent (Machery-Nagel, Düren, Germany) according to the manufacturer’s protocol
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Invitrogen Illumina™ TotalPrep™ RNA Amplification Kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description SAMPLE 8
replicate 2
Data processing The data were normalized using quantile normalization with IlluminaGUI in R
 
Submission date Aug 28, 2020
Last update date Aug 19, 2023
Contact name Liang-Chuan Lai
E-mail(s) llai@ntu.edu.tw
Phone 886-2-23123456
Organization name National Taiwan University
Department Physiology
Street address 1 Jen-Ai Road, Sec 1
City Taipei
State/province Taiwan
ZIP/Postal code 10051
Country Taiwan
 
Platform ID GPL10558
Series (1)
GSE157062 Microarray analysis of lung cancer cells gene expression in overexpressing different semaphorin 5a domains

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1343291 13.81794038 0
ILMN_1343295 12.89398722 0
ILMN_1651199 6.597076019 0.63507
ILMN_1651209 6.585359229 0.7026
ILMN_1651210 6.481837195 0.98701
ILMN_1651221 6.658094216 0.30649
ILMN_1651228 13.17698952 0
ILMN_1651229 7.9025438 0
ILMN_1651230 6.605929226 0.58312
ILMN_1651232 6.714901906 0.1
ILMN_1651235 6.670178119 0.23896
ILMN_1651236 6.497224807 0.97792
ILMN_1651237 8.979145841 0
ILMN_1651238 6.673949242 0.23117
ILMN_1651249 6.679590796 0.20649
ILMN_1651253 6.612144784 0.56234
ILMN_1651254 9.009964287 0
ILMN_1651259 6.764259713 0.04156
ILMN_1651260 6.591980332 0.67013
ILMN_1651262 12.27907558 0

Total number of rows: 47323

Table truncated, full table size 1446 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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