|
| Status |
Public on Sep 24, 2011 |
| Title |
KB2 (Illumina Mouse -6 V1.1) |
| Sample type |
RNA |
| |
|
| Source name |
KM12M cells xenografted into mouse brain
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
cell type: Human cancer KM12M cells xenografted into mouse brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissues with mirVana RNA Isolation labeling kit (Ambion Inc) according to the manufacturer protocol. RNA quality and integrity were confirmed by ExperionTM Automated Electrophoresis System (BIO-RAD)
|
| Label |
Biotin
|
| Label protocol |
Biotin-labeled cRNA samples for hybridization were prepared according to Illumina's recommended sample labeling procedure: 500 ng of total RNA was used for cDNA synthesis, followed by an amplification/labeling step (in vitro transcription) to synthesize biotin-labeled cRNA using the Illumina TotalPrep RNA Amplification kit (Ambion Inc., Austin, TX). cRNA concentrations using spectrophotometer (Nanodrop, ND-1000)
|
| |
|
| Hybridization protocol |
Labeled, amplified material (750 ng per array) was hybridized to the Illumina Mouse-6 BeadChip v.1.1(46K) according to the Manufacturer's instructions (Illumina, Inc., San Diego, CA).
|
| Scan protocol |
Arrays were scanned with an Illumina Bead array Reader confocal scanner (BeadStation 500GXDW; Illumina, Inc., San Diego, CA) according to the Manufacturer's instructions
|
| Description |
KM12M cells xenografted into mouse brain
To export to gene expression data based on unique gene, we used Sample Gene Profile option of Illumina BeadStudio software. The whole 46628 probes on the Mouse-6 beadChip ver. 1.1 were used.
|
| Data processing |
Array data processing were performed on Illumina BeadStudio software. Quantile normalization.
|
| |
|
| Submission date |
Nov 25, 2009 |
| Last update date |
Sep 24, 2011 |
| Contact name |
Sangbae Kim |
| E-mail(s) |
sangbkim22@gmail.com
|
| Organization name |
Baylor Colleage of Medicine
|
| Department |
HGSC
|
| Street address |
1 Baylor plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6568 |
| Series (1) |
| GSE19184 |
Cross species hybridization of human cancer cells xenografted into different mouse organ sites |
|
