|
| Status |
Public on Feb 01, 2010 |
| Title |
Memory B cells_M618 |
| Sample type |
RNA |
| |
|
| Source name |
Memory B cells isolated from tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD27-positive CD10/CD38-negative B-cells purified by magnetic cell separation
|
| Treatment protocol |
Upon magnetic cell sorting cells were lysated in Trizol Reagent (Invitrogen) and frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's indications.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for at least 16 hr at 45C on GeneChip Human Genome Array U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from human memory B cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 30, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Katia Basso |
| E-mail(s) |
kb451@columbia.edu
|
| Phone |
212-8515269
|
| Fax |
212-8515256
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1150 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12195 |
Mutations of multiple genes deregulate the NF-kB pathway in diffuse large B cell lymphoma |
|
| Relations |
| Reanalyzed by |
GSE86362 |
