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Sample GSM478467 Query DataSets for GSM478467
Status Public on Aug 15, 2013
Title Liver metastasis, 3 (U133A)
Sample type RNA
 
Source name Liver metastasis
Organism Homo sapiens
Characteristics tissue: liver metastasis
Growth protocol Freshly frozen PDACs, metastatic liver and normal pancreas samples were obtained with full ethical approval from the host institutions.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tissues with TRIzol Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol. Total RNA from normal liver was purchased from Ambion (Invitrogen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000.
Description Gene expression data from Liver Metastasis.
21m_a
Data processing Robust multi-array analysis (RMA) using R.
 
Submission date Dec 02, 2009
Last update date Aug 16, 2013
Contact name Claude Chelala
E-mail(s) c.chelala@qmul.ac.uk
Organization name Barts & The London School of Medicine (QMUL)
Department Institute of Cancer
Lab Centre for Molecular Oncology & Imaging
Street address Charterhouse square
City London
State/province London
ZIP/Postal code EC1M6BQ
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE19279 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133A]
GSE19281 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells

Data table header descriptions
ID_REF
VALUE RMA-normalized intensity value (log-scale)

Data table
ID_REF VALUE
1007_s_at 8.893852389
1053_at 4.446551645
117_at 5.070825978
121_at 7.521437542
1255_g_at 3.423791424
1294_at 6.223101728
1316_at 5.248843923
1320_at 4.196986576
1405_i_at 4.512753703
1431_at 7.822189309
1438_at 5.679460913
1487_at 6.955489368
1494_f_at 8.420877337
1598_g_at 8.856663891
160020_at 7.286110062
1729_at 6.626667192
1773_at 4.258238316
177_at 4.598849425
179_at 8.10302485
1861_at 4.451754671

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM478467.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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