|
| Status |
Public on Aug 15, 2013 |
| Title |
Liver metastasis, 2 (U133B) |
| Sample type |
RNA |
| |
|
| Source name |
Liver metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver metastasis
|
| Growth protocol |
Freshly frozen PDACs, metastatic liver and normal pancreas samples were obtained with full ethical approval from the host institutions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissues with TRIzol Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol. Total RNA from normal liver was purchased from Ambion (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
Gene expression data from Liver Metastasis.
22m_b
|
| Data processing |
Robust multi-array analysis (RMA) using R.
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 16, 2013 |
| Contact name |
Claude Chelala |
| E-mail(s) |
c.chelala@qmul.ac.uk
|
| Organization name |
Barts & The London School of Medicine (QMUL)
|
| Department |
Institute of Cancer
|
| Lab |
Centre for Molecular Oncology & Imaging
|
| Street address |
Charterhouse square
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
EC1M6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL97 |
| Series (2) |
| GSE19280 |
S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133B] |
| GSE19281 |
S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells |
|
