|
| Status |
Public on Feb 18, 2021 |
| Title |
Rhesus Macaque lumbar cord with West Nile virus neuroinvasive disease 3 days post infection replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Rhesus Macaque lumbar cord with West Nile virus neuroinvasive disease from 1999 outbreak in New York 3 days post infection replicate 3
|
| Organism |
Macaca mulatta |
| Characteristics |
time point: 3 days post infection
tissue: lumbar cord
infection: WNV(NY99)
|
| Treatment protocol |
Rhesus monkeys (Macaca mulatta; WNV-seronegative; 2–3-year-old) were inoculated intrathalamically (bilaterally) with a dose of 5.0 log10 PFU of wild-type WNV strain NY99-35262. CNS tissue from these animals was systematically collected for downstream analyses
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The miRNeasy Mini kit (Qiagen) was used to extract total RNA
|
| Label |
biotin
|
| Label protocol |
The cDNA (3.75 μg) was fragmented and labeled using the Encore Biotin Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, samples were hybridized for 16 hr at 45C on GeneChip Rhesus Macaque.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G Scanner.
|
| Description |
Gene expression data from Rhesus Macaque lumbar cord with West Nile virus neuroinvasive disease from 1999 outbreak in New York 3 days post infection replicate 3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
|
| |
|
| Submission date |
Sep 18, 2020 |
| Last update date |
Feb 18, 2021 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE122798 |
Virus infection of the CNS disrupts the immune-neural-synaptic axis via induction of pleiotropic gene regulation of host responses |
|
