|
| Status |
Public on Feb 24, 2021 |
| Title |
w20-hsh-day10-replicate-1 |
| Sample type |
SRA |
| |
|
| Source name |
w20-hsh-day10
|
| Organism |
Mus musculus |
| Characteristics |
cell line/type: W20 cells in osteogenic differentiation medium
genotype/variation: Elavl1 shRNA
|
| Treatment protocol |
short hairpin RNA knockdown W20 MSCs were cultured in osteogenic differentiation medium for 10 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
TRA00078371
78371L001
|
| Data processing |
NOT PROVIDED; REQUESTED
Supplementary_files_format_and_content: gene_exp.diff is the resulting differential expression results(1.5 fold) for control shRNA vs. Elavl1 shRNA. This file is output by cuffdiff. A q-value cutoff of 0.05 was used to determine differentially expressed genes.
|
| |
|
| Submission date |
Sep 20, 2020 |
| Last update date |
Feb 24, 2021 |
| Contact name |
Satya Kota |
| Organization name |
Harvard School of Dental Medicine
|
| Street address |
188 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE158260 |
Gene expression analysis using RNA-seq of control and Elavl1 knockdown W20 cells during osteogenic differentiation |
|
| Relations |
| BioSample |
SAMN16226941 |
| SRA |
SRX9158871 |
