|
| Status |
Public on Jun 16, 2010 |
| Title |
MMTV-Wnt/ILK tumor rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary tumor tissue from double-transgenic MMTV-Wnt/ILK mouse.
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary fat pad
gender: female
parity: nulliparous
transgenic strain: MMTV-Wnt/ILK
|
| Growth protocol |
Nulliparous females were monitored bi-weekly for the appearance of a small palpable tumor nodule in the mammary fat pads.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1 μg of RNA was used as starting material and rRNA reduced using the RiboMinus Transcriptome Isolation Kit (Invitrogen) as per Affymetrix recommendations.
|
| Label |
biotin
|
| Label protocol |
Total RNA was extracted from snap frozen tumor tissue using the GeneChip Whole Transcript (WT) Sense Target (ST) Labeling Assay (Affymetrix) as per manufacturer’s recommendations before being hybridized to a GeneChip Mouse Exon 1.0 ST Array (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the CTAG Facility of the British Columbia Cancer Agency, Vancouver, BC, Canada.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instructions (Affymetrix)
|
| Description |
RMA expression value derived from ArrayAssist version 5.0 software; core-exon and gene level analysis
|
| Data processing |
CEL files were processed using ArrayAssist 5.0. Targets showing absolute fold change greater than 1.5 and p-value < 0.05 were assessed for further investigation.
|
| |
|
| Submission date |
Dec 03, 2009 |
| Last update date |
Jun 16, 2010 |
| Contact name |
Steven McKinney |
| E-mail(s) |
smckinney@bccrc.ca
|
| Phone |
604-675-8000
|
| Organization name |
British Columbia Cancer Agency
|
| Department |
Molecular Oncology
|
| Lab |
Samuel Aparicio
|
| Street address |
675 West 10th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE19300 |
Activation of ER-alpha network in MMTV-Wnt/ILK mammary tumors |
|
