|
| Status |
Public on Nov 03, 2021 |
| Title |
C. albicans WT rep1 |
| Sample type |
SRA |
| |
|
| Source name |
WT-1
|
| Organism |
Candida albicans |
| Characteristics |
genotype: clinical isolate
|
| Growth protocol |
C. albicans strains were grown in YPD media (1% yeast extracts, 2% peptone, 2% glucose) at 30˚C. Overnigh cultured cells were diluted to OD600 of 0.1 into fresh 10ml YPD and grown at 30˚C until OD600 of 1.0.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using NucleoSpin® RNA (MN, MN740955) according to the protocol of the manufacturer. For sequencing, mRNA was captured using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, E7490).
A strand-specific sequencing library was synthesized using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (NEB, E7420) according to the instruction manual.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
The Illumina Hiseq generates raw images utilizing HCS(HiSeq Control Software v2.2) for system control
Base calling through an integrated primary analysis software called RTA(Real Time Analysis. v1.18)
The BCL(base calls) binary is converted into FASTQ utilizing illumina package bcl2fastq(v1.8.4)
Adapters are not trimmed away from the reads.
Genome_build: Candida albicans SC5314 (PRJNA10701)
|
| |
|
| Submission date |
Sep 24, 2020 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Jueun Kim |
| Organization name |
Kangwon National University
|
| Street address |
1 Kangwondaehak-gil
|
| City |
Chuncheon-si, Gangwon-do |
| ZIP/Postal code |
24341 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19036 |
| Series (1) |
| GSE158481 |
Differentially expressed genes in absence of histone H3K4 methyltransferase Set1 in Candida albicans |
|
| Relations |
| BioSample |
SAMN16253240 |
| SRA |
SRX9182647 |
