|
| Status |
Public on Dec 09, 2009 |
| Title |
H3R17me2_ChIP_Estrogen_ArrayF |
| Sample type |
genomic |
| |
|
| Source name |
H3R17me2 ChIP
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: MCF7 breast cancer cells
antibody: H3K17me2
agent: Estrogen
|
| Treatment protocol |
control (ethanol) or estrogen (E2: 1x10-8M) for 45 minutes
|
| Growth protocol |
phenol-red free DMEM supplemented with 10% charcoal dextran treated serum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
according to (Carroll et al 2005)
|
| Label |
biotin
|
| Label protocol |
according to (Carroll et al 2005)
|
| |
|
| Hybridization protocol |
according to (Carroll et al 2005)
|
| Scan protocol |
according to (Carroll et al 2005)
|
| Description |
H3R17me2_ChIP_Estrogen_ArrayF
|
| Data processing |
CEL files analyzed with MAT algorithm
|
| |
|
| Submission date |
Dec 04, 2009 |
| Last update date |
Dec 08, 2009 |
| Contact name |
Mathieu Lupien |
| E-mail(s) |
mathieu.lupien@dartmouth.edu
|
| Phone |
603-653-9041
|
| Organization name |
Norris Cotton Cancer Center/Dartmouth Medical School
|
| Department |
Genetics
|
| Street address |
One Medical Center Drive
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL4915 |
| Series (1) |
| GSE19328 |
Coativator Function Defines the Active Estrogen Receptor Alpha Cistrome |
|
