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| Status |
Public on Apr 17, 2021 |
| Title |
Testis-1_A01_180202 |
| Sample type |
SRA |
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| Source name |
Testis
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| Organism |
Macaca mulatta |
| Characteristics |
tissue: Testis
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the Trizol Reagent (Invitrogen, catalog #15596018) and analyzed on an Agilent 2100 Bioanalyzer to access the quality. The quantity of total RNA was measured by Qubit. cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Clotech, catalog #634925) and PrimeSTAR GXL DNA Polymerase (Clontech, catalog #R050B).
SMRT bell libraries were generated by using SMRTbellTM Template prep kit 1.0-SPv3 (PN 100-991-900)
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Sequel |
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| Description |
testis.uniq.sorted.bw
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| Data processing |
Library strategy: Iso-seq
ROI (Reads-Of-Insert) reads were extracted and primer sequences flanking ROI reads were then trimmed
Primer-trimmed reads were further processed to scan the PA tail and trimmed to get PA-trimmed reads which represents reads with a PA tail trimmed.
PA-trimmed reads were then mapped to the reference genome (rheMac8 for macaque; mm9 for mouse) by GMAP and the low-quality reads were filtered to get the final processed alignments.
Genome_build: rheMac8, mm9
Supplementary_files_format_and_content: bigWig files were generated using DeepTools (version 3.3.0) with default parameters; scores represented coverage of uniquely-mapped Iso-seq reads；
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| Submission date |
Sep 28, 2020 |
| Last update date |
Apr 17, 2021 |
| Contact name |
Qi Trinity Peng |
| E-mail(s) |
pengqi@pku.edu.cn
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| Organization name |
Peking University
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| Department |
Institute of Molecular Medicine
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| Street address |
No.5 Yiheyuan Road Haidian District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platform ID |
GPL26751 |
| Series (1) |
| GSE158668 |
De novo assembly of macaque gene models |
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| Relations |
| BioSample |
SAMN16282340 |
| SRA |
SRX9203925 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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